Expression of adenovirus E1B mutant phenotypes is dependent on the host cell and on synthesis of E1A proteins

White, E., Stillman, B. (1987) Expression of adenovirus E1B mutant phenotypes is dependent on the host cell and on synthesis of E1A proteins. Journal of Virology, 61 (2). pp. 426-35. ISSN 0022-538X

URL: http://www.ncbi.nlm.nih.gov/pubmed/2949088

Abstract

Adenovirus mutants containing genetic alterations in the gene encoding the E1B 19,000-molecular-weight (19K) tumor antigen induce the degradation of host cell chromosomal DNA (deg phenotype) and enhanced cytopathic effect (cyt phenotype) after infection of HeLa and KB cells. The deg and cyt phenotypes are a consequence of viral early gene expression in the absence of the E1B 19K protein. The role of the E1A proteins in induction of the cyt and deg phenotypes was investigated by constructing E1A-E1B double mutant viruses. Viruses were constructed to express the individual E1A 13S, 12S, or 9S cDNA genes in the presence of a mutation in the gene encoding the E1B 19K tumor antigen. Expression of either the 13S or 12S E1A proteins in the absence of functional E1B 19K protein produced the deg and cyt phenotypes. In contrast, a virus which expressed exclusively the 9S E1A gene product in the absence of the E1B 19K gene product did not induce the deg and cyt phenotypes, even at high multiplicities of infection. Therefore, both the 13S and 12S E1A gene products could directly or indirectly cause the deg and cyt phenotypes during infection of HeLa cells with an E1B 19K gene mutant virus. Furthermore, the deg phenotype was found to be host cell type specific, occurring in HeLa and KB cells but not in growth-arrested human WI38 cells. These results indicate that expression of the E1A trans-activating and transforming proteins is necessary for the induction of the cyt and deg phenotypes and that host cell factors also play a role.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Adenovirus Early Proteins Adenoviruses Human genetics Cell Line DNA Replication DNA Viral genetics Genes Genes Viral Hela Cells Humans KB Cells Mutation Oncogene Proteins Viral biosynthesis genetics Phenotype Plasmids
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 17 Feb 2012 19:44
Last Modified: 20 Jun 2017 20:32
PMCID: PMC253966
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25141

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