Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro

Waga, S., Stillman, B. (1994) Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro. Nature, 369 (6477). pp. 207-12. ISSN 0028-0836 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7910375
DOI: 10.1038/369207a0

Abstract

Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-alpha/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-delta to replicate both strands of the double helix conjointly.

Item Type: Paper
Uncontrolled Keywords: Animals Cattle DNA Replication DNA Viral biosynthesis genetics DNA-Binding Proteins metabolism DNA-Directed DNA Polymerase metabolism Homeodomain Proteins Humans Nuclear Proteins metabolism Proliferating Cell Nuclear Antigen Proto-Oncogene Proteins c-bcl-2 Replication Factor A Replication Factor C Repressor Proteins Saccharomyces cerevisiae Proteins Simian virus 40 genetics Templates Genetic Viral Proteins metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 17 Feb 2012 20:44
Last Modified: 20 Jun 2017 19:49
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25130

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