Independent Mutations in Adenovirus Type 2 Ts-111 Cause Degradation of Cellular DNA and Defective Viral DNA Replication

Stillman, B. W., White, E., Grodzicker, T. (1984) Independent Mutations in Adenovirus Type 2 Ts-111 Cause Degradation of Cellular DNA and Defective Viral DNA Replication. Journal of Virology, 50 (2). pp. 598-605.

URL: http://www.ncbi.nlm.nih.gov/pubmed/6368865

Abstract

An adenovirus type 2 mutant, Ad2ts111, has previously been shown to be temperature-sensitive for viral DNA replication in vivo and also to induce degradation of cellular DNA. Soluble nuclear extracts prepared from Ad2ts111-infected human cervical carcinoma HeLa cells grown at either the permissive (32.degree. C) or the nonpermissive (39.5.degree. C) temperature are thermolabile for elongation but not for initiation of DNA replication in vitro. Adenovirus single-stranded-DNA-binding protein purified from wild-type-infected cells can complement these extracts at the restrictive temperature in vitro. The DNA-binding protein synthesized in Ad2ts111-infected cells is stable at the nonpermissive temperature and is phosphorylated, as is the wild-type protein. The mutant DNA-binding protein synthesized in Ad5ts125-infected cells is unstable. Ad2ts111 and Ad5ts125 do not complement each other for virus growth in vivo. Evidently, Ad2ts111 contains a mutation the DNA-binding protein that affects viral DNA synthesis. Unlike viral DNA synthesis, the induction of cellular DNA degradation in early region 1 on the virus genome. Thus, the 2 phenotypes displayed in Ad2ts111-infected cells, namely, the temperature-sensitive replication of viral DNA and the degradation of cell DNA, are the result of 2 separate mutations.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 29 Feb 2012 14:23
Last Modified: 20 Jun 2017 20:38
PMCID: PMC255680
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25110

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