An adenovirus protein associated with the ends of replicating DNA molecules

Stillman, B. W., Bellett, A. J. D. (1979) An adenovirus protein associated with the ends of replicating DNA molecules. Virology, 93 (1). pp. 69-79.

URL: http://www.ncbi.nlm.nih.gov/pubmed/433158
DOI: 10.1016/0042-6822(79)90276-9

Abstract

The adenovirus DNA-protein complex contains the linear DNA molecule and a 55K protein covalently attached to each 5' end. We describe a simple assay for adenovirus DNA-protein complex, in which DNA, covalently linked to the terminal protein, specifically binds to benzoylated naphthoylated DEAE-cellulose. The DNA-protein complex can be recovered intact from the column by elution with urea and SDS, or the DNA moiety can be eluted after incubation of the column with protease. Using this assay system, we have shown that protein is associated with pulse-labeled single strands of DNA from the terminal restriction enzyme fragments of replicating adenovirus DNA. We suggest that the terminal protein has a function in DNA replication.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 28 Feb 2012 20:08
Last Modified: 20 Jun 2017 20:47
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25102

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