The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro

Prelich, G., Kostura, M., Marshak, D. R., Mathews, M. B., Stillman, B. (1987) The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro. Nature, 326 (6112). pp. 471-475. ISSN 0028-0836 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2882422
DOI: 10.1038/326471a0

Abstract

Cell-free extracts prepared from human 293 cells, supplemented with purified SV40 large-T antigen, support replication of plasmids containing the SV40 origin of DNA replication. A cellular protein (Mr approximately 36,000) that is required for efficient SV40 DNA synthesis in vitro has been purified from these extracts. This protein is recognized by human autoantibodies and is identified as the cell-cycle regulated protein known as proliferating cell nuclear antigen (PCNA) or cyclin.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Amino Acid Sequence Autoantigens Cell Cycle Cell Line CellFree System DNA Replication Humans Kinetics Nucleoproteins physiology Plasmids Proliferating Cell Nuclear Antigen Simian virus 40 genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 02 Mar 2012 18:25
Last Modified: 20 Jun 2017 20:32
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25041

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