Heterochromatin Dynamics in Mouse Cells: Interaction between Chromatin Assembly Factor 1 and HP1 Proteins

Murzina, N., Verreault, A., Laue, E., Stillman, B. (1999) Heterochromatin Dynamics in Mouse Cells: Interaction between Chromatin Assembly Factor 1 and HP1 Proteins. Molecular Cell, 4 (4). pp. 529-540.

URL: http://www.ncbi.nlm.nih.gov/pubmed/10549285
DOI: 10.1016/S1097-2765(00)80204-X

Abstract

Mechanisms contributing to the maintenance of heterochromatin in proliferating cells are poorly understood. We demonstrate that chromatin assembly factor 1 (CAF-1) binds to mouse HP1 proteins via an N-terminal domain of its p150 subunit, a domain dispensable for nucleosome assembly during DNA replication. Mutations in p150 prevent association with HP1 in heterochromatin in cells that are not in S phase and the formation of CAF-1-HP1 complexes in nascent chromatin during DNA replication in vitro. We suggest that CAF-1 p150 has a heterochromatin-specific function distinct from its nucleosome assembly function during S phase. Just before mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin concomitant with histone H3 phosphorylation. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 02 Mar 2012 18:57
Last Modified: 20 Jun 2017 19:25
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25029

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