Replicator dominance in a eukaryotic chromosome

Marahrens, Y., Stillman, B. (1994) Replicator dominance in a eukaryotic chromosome. Embo Journal, 13 (14). pp. 3395-400. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/8045266

Abstract

Replicators are genetic elements that control initiation at an origin of DNA replication (ori). They were first identified in the yeast Saccharomyces cerevisiae as autonomously replicating sequences (ARSs) that confer on a plasmid the ability to replicate in the S phase of the cell cycle. The DNA sequences required for ARS function on a plasmid have been defined, but because many sequences that participate in ARS activity are not components of chromosomal replicators, a mutational analysis of the ARS1 replicator located on chromosome IV of S. cerevisiae was performed. The results of this analysis indicate that four DNA elements (A, B1, B2 and B3) are either essential or important for ori activation in the chromosome. In a yeast strain containing two closely spaced and identical copies of the ARS1 replicator in the chromosome, only one is active. The mechanism of replicator repression requires the essential A element of the active replicator. This element is the binding site for the origin recognition complex (ORC), a putative initiator protein. The process that determines which replicator is used, however, depends entirely upon flanking DNA sequences.

Item Type: Paper
Uncontrolled Keywords: Chromosomes Fungal Comparative Study DNA Mutational Analysis DNA Replication genetics DNA Fungal biosynthesis genetics Regulatory Sequences Nucleic Acid genetics Saccharomyces cerevisiae genetics Structure-Activity Relationship
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 06 Mar 2012 14:36
Last Modified: 20 Jun 2017 19:49
PMCID: PMC395237
URI: http://repository.cshl.edu/id/eprint/25014

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