Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein

Iizuka, M., Stillman, B. (1999) Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein. Journal of Biological Chemistry, 274 (33). pp. 23027-34. ISSN 0021-9258

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URL: http://www.ncbi.nlm.nih.gov/pubmed/10438470
DOI: 10.1074/jbc.274.33.23027

Abstract

The origin recognition complex (ORC) is an initiator protein for DNA replication, but also effects transcriptional silencing in Saccharomyces cerevisiae and heterochromatin function in Drosophila. It is not known, however, whether any of these functions of ORC is conserved in mammals. We report the identification of a novel protein, HBO1 (histone acetyltransferase binding to ORC), that interacts with human ORC1 protein, the largest subunit of ORC. HBO1 exists as part of a multisubunit complex that possesses histone H3 and H4 acetyltransferase activities. A fraction of the relatively abundant HBO1 protein associates with ORC1 in human cell extracts. HBO1 is a member of the MYST domain family that includes S. cerevisiae Sas2p, a protein involved in control of transcriptional silencing that also has been genetically linked to ORC function. Thus the interaction between ORC and a MYST domain acetyltransferase is widely conserved. We suggest roles for ORC-mediated acetylation of chromatin in control of both DNA replication and gene expression.

Item Type: Paper
Uncontrolled Keywords: Acetyltransferases chemistry genetics metabolism Amino Acid Sequence Base Sequence DNA-Binding Proteins metabolism Hela Cells Histone Acetyltransferases Humans Molecular Sequence Data Origin Recognition Complex Protein Binding Saccharomyces cerevisiae metabolism Saccharomyces cerevisiae Proteins Sequence Homology Amino Acid Transcription Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > origin recognition complex
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 06 Mar 2012 16:13
Last Modified: 20 Jun 2017 19:23
Related URLs:
URI: http://repository.cshl.edu/id/eprint/24990

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