Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo

Hoek, M., Stillman, B. (2003) Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo. Proceedings of the National Academy of Sciences of the United States of America, 100 (21). pp. 12183-12188. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/14519857
DOI: 10.1073/pnas.1635158100

Abstract

De novo chromatin assembly maintains histone density on the daughter strands in the wake of the replication fork. The heterotrimer chromatin assembly factor 1 (CAF-1) couples DNA replication to histone deposition in vitro, but is not essential for yeast cell proliferation. Depletion of CAF-1 in human cell lines demonstrated that CAF-1 was required for efficient progression through S-phase. Cells lacking CAF-1 accumulated in early and mid S-phase and replicated DNA slowly. The checkpoint kinase Chk1, but not Chk2, was phosphorylated in response to CAF-1 depletion, consistent with a DNA replication defect. CAF-1-depleted cell extracts completely lacked DNA replication-coupled chromatin assembly activity, suggesting that CAF-1 is required for efficient S-phase progression in human cells. These results indicate that, in contrast to yeast, human CAF-1 is necessary for coupling chromatin assembly with DNA replication.

Item Type: Paper
Uncontrolled Keywords: NEWLY SYNTHESIZED HISTONES newly synthesized histones CYCLIN-DEPENDENT KINASES cyclin dependent kinases CELL NUCLEAR cell nuclear ANTIGEN antigen S-PHASE PROGRESSION S-Phase progression S phase SACCHAROMYCES-CEREVISIAE CAF-1 Saccharomyces Cerevisiae MAMMALIAN-CELLS Mammalian Cells IONIZING-RADIATION Ionizing radiation DAMAGE CHECKPOINT damage checkpoint HIR PROTEINS YEAST HIR1P Yease
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 06 Mar 2012 16:04
Last Modified: 20 Jun 2017 18:18
PMCID: PMC218733
Related URLs:
URI: http://repository.cshl.edu/id/eprint/24988

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