A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria

Diffley, J. F., Stillman, B. (1991) A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria. Proceedings of the National Academy of Sciences of the United States of America, 88 (17). pp. 7864-8. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/1881919
DOI: 10.1073/pnas.88.17.7864

Abstract

ABF2 (ARS-binding factor 2), a small, basic DNA-binding protein that binds specifically to the autonomously replicating sequence ARS1, is located primarily in the mitochondria of the yeast Saccharomyces cerevisiae. The abundance of ABF2 and the phenotype of abf2- null mutants argue that this protein plays a key role in the structure, maintenance, and expression of the yeast mitochondrial genome. The predicted amino acid sequence of ABF2 is closely related to the high-mobility group proteins HMG1 and HMG2 from vertebrate cell nuclei and to several other DNA-binding proteins. Additionally, ABF2 and the other HMG-related proteins are related to a globular domain from the heat shock protein hsp70 family. ABF2 interacts with DNA both nonspecifically and in a specific manner within regulatory regions, suggesting a mechanism whereby it may aid in compacting the mitochondrial genome without interfering with expression.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Chromosomes Fungal Cloning Molecular Comparative Study DNA Mitochondrial genetics DNA Binding Proteins genetics Fungal Proteins genetics Gene Library Heat-Shock Proteins genetics High Mobility Group Proteins genetics Mitochondria metabolism Molecular Sequence Data Phenotype Restriction Mapping Saccharomyces cerevisiae genetics Saccharomyces cerevisiae Proteins Sequence Homology Nucleic Acid Transcription Factors
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 07 Mar 2012 16:20
Last Modified: 20 Jun 2017 20:08
PMCID: PMC52404
Related URLs:
URI: http://repository.cshl.edu/id/eprint/24954

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