Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer

Diffley, J. F., Stillman, B. (1988) Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer. Proceedings of the National Academy of Sciences of the United States of America, 85 (7). pp. 2120-4. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/3281162

Abstract

Two factors that interact specifically with the chromosomal replicator, autonomously replicating sequence 1 (ARS1), were identified in whole-cell yeast extracts. One of these proteins, ARS binding factor I (ABF-I), was purified to homogeneity as a polypeptide of 135 kDa. ABF-I binds within a region previously shown to be essential for the function of ARS1 when yeast are grown under certain nutritional conditions, and deletion analysis presented here suggests that the ARS1 domain containing the ABF-I-binding site, domain B, is a complex element composed of at least two subdomains. ABF-I also binds within an ARS at the HMR silent mating locus. This ARS functions as a cis-acting transcriptional silencer, and the ABF-I-binding site within this ARS is important for both ARS and silencer function.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Binding Sites DNA, Fungal metabolism DNA-Binding Proteins isolation & purification metabolism Fungal Proteins isolation & purification metabolism Gene Expression Regulation Genes, Fungal Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae genetics Transcription Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
biotechnology > chromatography > protein purification
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 07 Mar 2012 16:09
Last Modified: 20 Jun 2017 20:31
PMCID: PMC279940
Related URLs:
URI: http://repository.cshl.edu/id/eprint/24952

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