The knockout mouse project

Austin, C. P., Battey, J. F., Bradley, A., Bucan, M., Capecchi, M., Collins, F. S., Dove, W. F., Duyk, G., Dymecki, S., Eppig, J. T., Grieder, F. B., Heintz, N., Hicks, G., Insel, T. R., Joyner, A., Koller, B. H., Lloyd, K. C. K., Magnuson, T., Moore, M. W., Nagy, A., Pollock, J. D., Roses, A. D., Sands, A. T., Seed, B., Skarnes, W. C., Snoddy, J., Soriano, P., Stewart, D. J., Stewart, F., Stillman, B., Varmus, H., Varticovski, L., Verma, I. M., Vogt, T. F., von Melchner, H., Witkowski, J., Woychik, R. P., Wurst, W., Yancopoulos, G. D., Young, S. G., Zambrowicz, B. (2004) The knockout mouse project. Nature Genetics, 36 (9). pp. 921-924. ISSN 1061-4036

URL: http://www.ncbi.nlm.nih.gov/pubmed/15340423
DOI: 10.1038/ng0904-921

Abstract

Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes. Furthermore, many of these are limited in utility because they have not been made or phenotyped in standardized ways, and many are not freely available to researchers. It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain.

Item Type: Paper
Uncontrolled Keywords: EMBRYONIC STEM-CELLS embryonic stem cells GENE-TRAP MUTAGENESIS gene trap mutagenesis HUMAN GENOME human genome MICE mouse mice SEQUENCE sequence IDENTIFICATION identification DEFICIENCY deficiency PHENOTYPE phenotype RESOURCE resource INSIGHTS insights
Subjects: bioinformatics > genomics and proteomics > databases > database construction
bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 07 Mar 2012 18:59
Last Modified: 20 Jun 2017 18:15
PMCID: PMC2716027
Related URLs:
URI: http://repository.cshl.edu/id/eprint/24933

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