CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription

Yuan, C. C., Zhao, X., Florens, L., Swanson, S. K., Washburn, M. P., Hernandez, N. (December 2007) CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription. Mol Cell Biol, 27 (24). pp. 8729-8738. ISSN 0270-7306 (Print)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/17938208
DOI: 10.1128/mcb.00846-07

Abstract

Chromatin remodeling and histone modification are essential for eukaryotic transcription regulation, but little is known about chromatin-modifying activities acting on RNA polymerase III (pol III)-transcribed genes. The human U6 small nuclear RNA (snRNA) promoter, located 5' of the transcription start site, consists of a core region directing basal transcription and an activating region that recruits the transcription factors Oct-1 and Staf (ZNF143). Oct-1 activates transcription in part by helping recruit core binding factors, but nothing is known about the mechanisms of transcription activation by Staf. We show that Staf activates U6 transcription from a preassembled chromatin template in vitro and associates with several proteins linked to chromatin modification, among them the chromodomain-helicase-DNA binding protein 8 (CHD8). CHD8 binds to histone H3 di- and tri- methylated on lysine 4. It resides on the human U6- as well as the mRNA IRF3- promoters in vivo, and contributes to efficient transcription from both these promoters. Thus pol III transcription from type 3 promoters uses some of the same factors used for chromatin remodeling at pol II promoters.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: CSHL Librarian
Date: December 2007
Date Deposited: 01 Nov 2011 18:26
Last Modified: 11 Apr 2018 15:13
PMCID: PMC2169411
Related URLs:
URI: http://repository.cshl.edu/id/eprint/23180

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