Yuan, C. C., Zhao, X., Florens, L., Swanson, S. K., Washburn, M. P., Hernandez, N. (December 2007) CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription. Mol Cell Biol, 27 (24). pp. 8729-8738. ISSN 0270-7306 (Print)
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CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription.pdf Download (712kB) | Preview |
Abstract
Chromatin remodeling and histone modification are essential for eukaryotic transcription regulation, but little is known about chromatin-modifying activities acting on RNA polymerase III (pol III)-transcribed genes. The human U6 small nuclear RNA (snRNA) promoter, located 5' of the transcription start site, consists of a core region directing basal transcription and an activating region that recruits the transcription factors Oct-1 and Staf (ZNF143). Oct-1 activates transcription in part by helping recruit core binding factors, but nothing is known about the mechanisms of transcription activation by Staf. We show that Staf activates U6 transcription from a preassembled chromatin template in vitro and associates with several proteins linked to chromatin modification, among them the chromodomain-helicase-DNA binding protein 8 (CHD8). CHD8 binds to histone H3 di- and tri- methylated on lysine 4. It resides on the human U6- as well as the mRNA IRF3- promoters in vivo, and contributes to efficient transcription from both these promoters. Thus pol III transcription from type 3 promoters uses some of the same factors used for chromatin remodeling at pol II promoters.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Stenlund lab |
| Depositing User: | CSHL Librarian |
| Date: | December 2007 |
| Date Deposited: | 01 Nov 2011 18:26 |
| Last Modified: | 11 Apr 2018 15:13 |
| PMCID: | PMC2169411 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/23180 |
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