Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections

Petreanu, L. T., Huber, D., Sobczyk, A., Svoboda, K. (May 2007) Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections. Nat Neurosci, 10 (5). pp. 663-668. ISSN 1097-6256

URL: https://www.ncbi.nlm.nih.gov/pubmed/17435752
DOI: 10.1038/nn1891

Abstract

The functions of cortical areas depend on their inputs and outputs, but the detailed circuits made by long- range projections are unknown. We show that the light- gated channel channelrhodopsin- 2 ( ChR2) is delivered to axons in pyramidal neurons in vivo. In brain slices from ChR2- expressing mice, photostimulation of ChR2- positive axons can be transduced reliably into single action potentials. Combining photostimulation with whole- cell recordings of synaptic currents makes it possible to map circuits between presynaptic neurons, defined by ChR2 expression, and postsynaptic neurons, defined by targeted patching. We applied this technique, ChR2- assisted circuit mapping ( CRACM), to map long- range callosal projections from layer ( L) 2/3 of the somatosensory cortex. L2/3 axons connect with neurons in L5, L2/3 and L6, but not L4, in both ipsilateral and contralateral cortex. In both hemispheres the L2/3- to- L5 projection is stronger than the L2/3- to- L2/3 projection. Our results suggest that laminar specificity may be identical for local and longrange cortical projections.

Item Type: Paper
Uncontrolled Keywords: VISUAL-CORTEX SYNAPTIC CONNECTIONS SOMATOSENSORY CORTEX FLUORESCENT PROTEIN CORTICAL CIRCUITS CORPUS-CALLOSUM OPTICAL CONTROL RAT NEOCORTEX ADULT-RAT NEURONS
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: CSHL Librarian
Date: May 2007
Date Deposited: 07 Nov 2011 20:56
Last Modified: 02 Apr 2018 20:28
Related URLs:
URI: http://repository.cshl.edu/id/eprint/23119

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