A mammalian microRNA expression atlas based on small RNA library sequencing

Landgraf, P., Rusu, M., Sheridan, R., Sewer, A., Iovino, N., Aravin, A. A., Pfeffer, S., Rice, A., Kamphorst, A. O., Landthaler, M., Lin, C., Socci, N. D., Hermida, L., Fulci, V., Chiaretti, S., Foa, R., Schliwka, J., Fuchs, U., Novosel, A., Muller, R. U., Schermer, B., Bissels, U., Inman, J., Phan, Q., Chien, M. C., Weir, D. B., Choksi, R., De Vita, G., Frezzetti, D., Trompeter, H. I., Hornung, V., Teng, G., Hartmann, G., Palkovits, M., Di Lauro, R., Wernet, P., Macino, G., Rogler, C. E., Nagle, J. W., Ju, J. Y., Papavasiliou, F. N., Benzing, T., Lichter, P., Tam, W., Brownstein, M. J., Bosio, A., Borkhardt, A., Russo, J. J., Sander, C., Zavolan, M., Tuschl, T. (June 2007) A mammalian microRNA expression atlas based on small RNA library sequencing. Cell, 129 (7). pp. 1401-1414. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/17604727
DOI: 10.1016/j.cell.2007.04.040

Abstract

MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.

Item Type: Paper
Uncontrolled Keywords: MENTAL-RETARDATION PROTEIN POSTTRANSCRIPTIONAL REGULATION C-ELEGANS CAENORHABDITIS-ELEGANS/ ANIMAL DEVELOPMENT GENE-EXPRESSION IDENTIFICATION TARGETS MIRNAS ZEBRAFISH
Subjects: organism description > animal > C elegans
bioinformatics > genomics and proteomics > annotation > map annotation
bioinformatics > computational biology
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

therapies > stem cells
organism description > animal > fish > zebrafish
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: CSHL Librarian
Date: June 2007
Date Deposited: 10 Nov 2011 22:25
Last Modified: 30 Mar 2018 15:21
PMCID: PMC2681231
Related URLs:
URI: http://repository.cshl.edu/id/eprint/23076

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