A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells

Ibarra, I., Erlich, Y., Muthuswamy, S. K., Sachidanandam, R., Hannon, Gregory J. (December 2007) A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells. Genes Dev, 21 (24). pp. 3238-43. ISSN 0890-9369 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/18079172
DOI: 10.1101/gad.1616307

Abstract

microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial cell line, Comma-Dbeta, contains a population of self-renewing progenitor cells that can reconstitute the mammary gland. We purified this population and determined its miRNA signature. Several microRNAs, including miR-205 and miR-22, are highly expressed in mammary progenitor cells, while others, including let-7 and miR-93, are depleted. Let-7 sensors can be used to prospectively enrich self-renewing populations, and enforced let-7 expression induces loss of self-renewing cells from mixed cultures.

Item Type: Paper
Additional Information: 0890-9369 (Print)
Uncontrolled Keywords: RNAi microRNA stem cell
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

organs, tissues, organelles, cell types and functions > tissues types and functions > mammary gland
organism description > animal > mammal > rodent > mouse
therapies > stem cells
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL labs > Muthuswamy lab
CSHL labs > Sachidanandam lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: 15 December 2007
Date Deposited: 14 Nov 2011 19:06
Last Modified: 22 Sep 2014 15:03
PMCID: PMC2113025
Related URLs:
URI: http://repository.cshl.edu/id/eprint/23046

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