The fission yeast Jmj2 reverses histone H3 lysine 4 trimethylation

Huarte, M., Lan, F., Kim, T. H., Vaughn, M. W., Zaratiegui, B. M., Martienssen, R. A., Buratowski, S., Shi, Y. (2007) The fission yeast Jmj2 reverses histone H3 lysine 4 trimethylation. Journal of Biological Chemistry, 282 (30). pp. 21662-21670. ISSN 0021-9258

URL: http://www.ncbi.nlm.nih.gov/pubmed/17550896
DOI: 10.1074/jbc.M703897200

Abstract

Histone methylation regulates transcription, chromatin structure, and the epigenetic state of the cell. Recent studies identified the JmjC domain as a catalytic module for histone demethylation. Schizosaccharomyces pombe contains seven JmjC proteins, but it was unclear whether any of them functioned as histone demethylases. In this report, we show that the JmjC protein Jmj2, which is evolutionarily conserved from yeast to human, reversed trimethylated H3-Lys-4 to di- and mono-but not unmethylated products. Overexpression of Jmj2 but not a catalytically inactive mutant reduced H3-Lys-4 trimethylation levels in vivo and suppressed the toxicity caused by overexpression of the H3-Lys-4-me3-binding protein Yng1 in budding yeast. Genome-wide analysis showed that the loss of jmj2 was associated with an increase in the H3-Lys-4-me3 signal, which was enriched near the transcriptional start sites and the coding regions. At the mating-type locus, the loss of jmj2 or substitution of jmj2 with a catalytically inactive form is correlated with increased reporter gene transcription and H3-Lys-4-me3/2 levels, suggesting that Jmj2 and its demethylase activity may play a role in heterochromatin biology. Our findings identified a novel S. pombe histone demethylase with specificity toward di- and trimethylated histone H3-Lys-4 and a possible role in heterochromatin regulation.

Item Type: Paper
Uncontrolled Keywords: DOMAIN-CONTAINING PROTEINS DNA-BINDING PROPERTIES METHYLATION PATTERNS DEMETHYLATION TRANSCRIPTION HETEROCHROMATIN FAMILY GENE DERNETHYLASE ACETYLATION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein demethylation
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: CSHL Librarian
Date Deposited: 14 Nov 2011 17:41
Last Modified: 09 Apr 2014 20:27
Related URLs:
URI: http://repository.cshl.edu/id/eprint/23043

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