A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in zebrafish

Houwing, S., Kamminga, L. M., Berezikov, E., Cronembold, D., Girard, A., van den Elst, H., Filippov, D. V., Blaser, H., Raz, E., Moens, C. B., Plasterk, R. H. A., Hannon, G. J., Draper, B. W., Ketting, R. F. (April 2007) A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in zebrafish. Cell, 129 (1). pp. 69-82. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/17418787
DOI: 10.1016/j.cell.2007.03.026

Abstract

Piwi proteins specify an animal-specific subclass of the Argonaute family that, in vertebrates, is specifically expressed in germ cells. We demonstrate that zebrafish Piwi (Ziwi) is expressed in both the male and the female gonad and is a component of a germline-specifying structure called nuage. Loss of Ziwi function results in a progressive loss of germ cells due to apoptosis during larval development. In animals that have reduced Ziwi function, germ cells are maintained but display abnormal levels of apoptosis in adults. In mammals, Piwi proteins associate with approximately 29-nucleotide-long, testis-specific RNA molecules called piRNAs. Here we show that zebrafish piRNAs are present in both ovary and testis. Many of these are derived from transposons, implicating a role for piRNAs in the silencing of repetitive elements in vertebrates. Furthermore, we show that piRNAs are Dicer independent and that their 3' end likely carries a 2'O-Methyl modification.

Item Type: Paper
Uncontrolled Keywords: SMALL RNAS PROCESSING BODIES DROSOPHILA SIRNAS MIGRATION PATHWAY PROTEIN TESTES MIWI SPERMATOGENESIS
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
organism description > animal > fish > zebrafish
CSHL Authors:
Communities: CSHL labs > Hannon lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: April 2007
Date Deposited: 15 Nov 2011 16:14
Last Modified: 22 Sep 2014 14:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/23037

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