Recurrent DNA copy number variation in the laboratory mouse

Egan, C. M., Sridhar, S., Wigler, M. H., Hall, I. M. (November 2007) Recurrent DNA copy number variation in the laboratory mouse. Nat Genet, 39 (11). pp. 1384-9. ISSN 1061-4036 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/17965714
DOI: 10.1038/ng.2007.19

Abstract

Different species, populations and individuals vary considerably in the copy number of discrete segments of their genomes. The manner and frequency with which these genetic differences arise over generational time is not well understood. Taking advantage of divergence among lineages sharing a recent common ancestry, we have conducted a genome-wide analysis of spontaneous copy number variation (CNV) in the laboratory mouse. We used high-resolution microarrays to identify 38 CNVs among 14 colonies of the C57BL/6 strain spanning approximately 967 generations of inbreeding, and we examined these loci in 12 additional strains. It is clear from our results that many CNVs arise through a highly nonrandom process: 18 of 38 were the product of recurrent mutation, and rates of change varied roughly four orders of magnitude across different loci. Recurrent CNVs are found throughout the genome, affect 43 genes and fluctuate in copy number over mere hundreds of generations, observations that raise questions about their contribution to natural variation.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > morphological divergence
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > spontaneous copy number variation
CSHL Authors:
Communities: CSHL labs > Wigler lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: November 2007
Date Deposited: 15 Nov 2011 16:34
Last Modified: 26 Sep 2014 15:00
Related URLs:
URI: http://repository.cshl.edu/id/eprint/23007

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