Nestin-GFP reporter expression defines the quiescent state of skeletal muscle satellite cells

Day, K., Shefer, G., Richardson, J. B., Enikolopov, G. N., Yablonka-Reuveni, Z. (April 2007) Nestin-GFP reporter expression defines the quiescent state of skeletal muscle satellite cells. Developmental Biology, 304 (1). pp. 246-259. ISSN 0012-1606

URL: https://www.ncbi.nlm.nih.gov/pubmed/17239845
DOI: 10.1016/j.ydbio.2006.12.026

Abstract

Repair of adult skeletal muscle depends on satellite cells, quiescent myogenic stem cells located beneath the myofiber basal lamina. Satellite cell numbers and performance decline with age and disease, yet the intrinsic molecular changes accompanying these conditions are unknown. We identified expression of GFP driven by regulatory elements of the nestin (NES) gene within mouse satellite cells, which permitted characterization of these cells in their niche. Sorted NES-GFP(+) cells exclusively acquired a myogenic fate, even when supplemented with media supporting nonmyogenic development. Mutual and unique gene expression by NES-GFP(+) cells from hindlimb and diaphragm muscles demonstrated intra- and inter-muscular heterogeneity of satellite cells. NES-GFP expression declined following satellite cell activation and was reacquired in late stage myogenic cultures by non-proliferating Pax7(+) progeny. The dynamics of this expression pattern reflect the cycle of satellite cell self-renewal. The NES-GFP model reveals unique transcriptional activity within quiescent satellite cells and permits novel insight into the heterogeneity of their molecular signatures. (c) 2006 Elsevier Inc. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: satellite cells self-renewal stem cells endothelial cells nestin desmin Myf5 Pax3 Pax7 Sca1 CD31 GFP Sca1-GFP nestin-GFP INTERMEDIATE-FILAMENT PROTEIN DYSTROPHIC MDX MICE PROGENITOR CELLS STEM-CELLS MYOGENIC PROGENITORS GENE-EXPRESSION SELF-RENEWAL NEURAL STEM DIFFERENTIATION REGENERATION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > nestin
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > satellite cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > satellite cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > satellite cell

therapies > stem cells
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: CSHL Librarian
Date: April 2007
Date Deposited: 30 Nov 2011 19:42
Last Modified: 01 Mar 2017 15:35
PMCID: PMC1888564
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22992

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