SR proteins function in coupling RNAP II transcription to pre-mRNA splicing

Das, R., Yu, J., Zhang, Z., Gygi, M. P., Krainer, A. R., Gygi, S. P., Reed, R. (June 2007) SR proteins function in coupling RNAP II transcription to pre-mRNA splicing. Molecular Cell, 26 (6). pp. 867-881. ISSN 1097-2765

URL: http://www.ncbi.nlm.nih.gov/pubmed/17588520
DOI: 10.1016/j.molcel.2007.05.036

Abstract

Transcription and splicing are functionally coupled, resulting in highly efficient splicing of RNA polymerase II (RNAP II) transcripts. The mechanism involved in this coupling is not known. To identify potential coupling factors, we carried out a comprehensive proteomic analysis of immunopurified human RNAP II, identifying > 100 specifically associated proteins. Among these are the SR protein family of splicing factors and all of the components of U1 snRNP, but no other snRNPs or splicing factors. We show that SR proteins function in coupling transcription to splicing and provide evidence that the mechanism involves cotranscriptional recruitment of SR proteins to RNAP II transcripts. We propose that the exclusive association of U1 snRNP/SR proteins with RNAP II positions these splicing factors, which are known to function early in spliceosome assembly, close to the nascent pre-mRNA. Thus, these factors readily out-compete inhibitory hnRNP proteins, resulting in efficient spliceosome assembly on nascent RNAP II transcripts.

Item Type: Paper
Uncontrolled Keywords: RS DOMAINS CONTACT C-TERMINAL DOMAIN POLYMERASE-II NUCLEAR-MATRIX IN-VITRO SPLICEOSOME COMPLEX CTD RECRUITMENT ENHANCER
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > SR proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: CSHL Librarian
Date: June 2007
Date Deposited: 30 Nov 2011 19:45
Last Modified: 09 Apr 2014 14:23
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22991

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