The central cell plays a critical role in pollen tube guidance in Arabidopsis

Chen, Y. H., Li, H. J., Shi, D. Q., Yuan, L., Liu, J., Sreenivasan, R., Baskar, R., Grossniklaus, U., Yang, W. C. (November 2007) The central cell plays a critical role in pollen tube guidance in Arabidopsis. Plant Cell, 19 (11). pp. 3563-3577. ISSN 1040-4651

URL: https://www.ncbi.nlm.nih.gov/pubmed/18055609
DOI: 10.1105/tpc.107.053967

Abstract

The sperm cell of flowering plants cannot migrate unaided and must be transported by the pollen tube cell of the male gametophyte to achieve successful fertilization. Long-distance pollen tube guidance is controlled by the seven-celled female gametophyte, the embryo sac. Previous reports showed that the synergid cell of the embryo sac is essential for pollen tube guidance. Here, we report the identification of a central cell guidance (ccg) mutant, which is defective in micropylar pollen tube guidance. CCG encodes a nuclear protein with an N-terminal conserved zinc beta-ribbon domain that is functionally interchangeable with that of TFIIB in yeast. This suggests that CCG might act as a transcription regulator for pollen tube guidance. CCG is expressed in the central cell of the female gametophyte. Expression of CCG in the central cell alone is sufficient to restore the normal pollen tube guidance phenotype, demonstrating that the central cell plays a critical role in pollen tube guidance.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: RNA-POLYMERASE-II TRANSCRIPTION FACTOR-TFIIB START SITE SELECTION VITRO STYLAR MATRIX ZINC RIBBON DOMAIN FUNCTIONAL DOMAINS IN-VITRO FEMALE GAMETOPHYTE FLOWERING PLANTS SEED DEVELOPMENT
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > gametophyte
organism description > plant
CSHL Authors:
Communities: CSHL labs > Grossniklaus lab
Depositing User: CSHL Librarian
Date: November 2007
Date Deposited: 02 Dec 2011 14:48
Last Modified: 22 Mar 2018 15:50
PMCID: PMC2174880
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22985

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