MIWI2 Is Essential for Spermatogenesis and Repression of Transposons in the Mouse Male Germline

Carmell, M. A., Girard, A., van de Kant, H. J. G., Bourc'his, D., Bestor, T. H., de Rooij, D. G., Hannon, G. J. (April 2007) MIWI2 Is Essential for Spermatogenesis and Repression of Transposons in the Mouse Male Germline. Developmental Cell, 12 (4). pp. 503-514.

URL: http://www.ncbi.nlm.nih.gov/pubmed/17395546
DOI: 10.1016/j.devcel.2007.03.001

Abstract

Summary Small RNAs associate with Argonaute proteins and serve as sequence-specific guides for regulation of mRNA stability, productive translation, chromatin organization, and genome structure. In animals, the Argonaute superfamily segregates into two clades. The Argonaute clade acts in RNAi and in microRNA-mediated gene regulation in partnership with 21-22 nt RNAs. The Piwi clade, and their 26-30 nt piRNA partners, have yet to be assigned definitive functions. In mice, two Piwi-family members have been demonstrated to have essential roles in spermatogenesis. Here, we examine the effects of disrupting the gene encoding the third family member, MIWI2. Miwi2-deficient mice display a meiotic-progression defect in early prophase of meiosis I and a marked and progressive loss of germ cells with age. These phenotypes may be linked to an inappropriate activation of transposable elements detected in Miwi2 mutants. Our observations suggest a conserved function for Piwi-clade proteins in the control of transposons in the germline.

Item Type: Paper
Uncontrolled Keywords: RNA DEVBIO DOUBLE-STRAND BREAKS CELL SELF-RENEWAL DROSOPHILA-MELANOGASTER SMALL RNAS STEM-CELLS MEIOTIC CHROMOSOMES PIWI MICE RETROTRANSPOSON GENE
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > PAZ protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Piwi protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
CSHL Authors:
Communities: CSHL labs > Hannon lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: April 2007
Date Deposited: 01 Dec 2011 21:59
Last Modified: 22 Sep 2014 14:33
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22980

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