Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila

Brennecke, J., Aravin, A. A., Stark, A., Dus, M., Kellis, M., Sachidanandam, R., Hannon, G. J. (2007) Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell, 128 (6). pp. 1089-103. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/17346786
DOI: 10.1016/j.cell.2007.01.043

Abstract

Drosophila Piwi-family proteins have been implicated in transposon control. Here, we examine piwi-interacting RNAs (piRNAs) associated with each Drosophila Piwi protein and find that Piwi and Aubergine bind RNAs that are predominantly antisense to transposons, whereas Ago3 complexes contain predominantly sense piRNAs. As in mammals, the majority of Drosophila piRNAs are derived from discrete genomic loci. These loci comprise mainly defective transposon sequences, and some have previously been identified as master regulators of transposon activity. Our data suggest that heterochromatic piRNA loci interact with potentially active, euchromatic transposons to form an adaptive system for transposon control. Complementary relationships between sense and antisense piRNA populations suggest an amplification loop wherein each piRNA-directed cleavage event generates the 5' end of a new piRNA. Thus, sense piRNAs, formed following cleavage of transposon mRNAs may enhance production of antisense piRNAs, complementary to active elements, by directing cleavage of transcripts from master control loci.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence DNA Transposable Elements/ genetics Drosophila Proteins/genetics/ metabolism Drosophila melanogaster/ genetics Female Genome, Insect Male Molecular Sequence Data Multigene Family Ovary/chemistry Peptide Initiation Factors/genetics/ metabolism Proteins/genetics/ metabolism RNA/biosynthesis/chemistry/genetics/ metabolism RNA, Antisense/chemistry/ metabolism RNA-Binding Proteins/genetics/metabolism Recombinant Proteins/genetics Retroelements Transcription Factors/genetics
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Piwi protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > antisense
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL labs > Sachidanandam lab
Watson School > Publications
Depositing User: CSHL Librarian
Date Deposited: 01 Dec 2011 21:28
Last Modified: 22 Sep 2014 14:15
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22977

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