A conserved role for kinesin-5 in plant mitosis

Bannigan, A., Scheible, W. R., Lukowitz, W., Fagerstrom, C., Wadsworth, P., Somerville, C., Baskin, T. I. (August 2007) A conserved role for kinesin-5 in plant mitosis. Journal of Cell Science, 120 (16). pp. 2819-2827. ISSN 0021-9533

URL: https://www.ncbi.nlm.nih.gov/pubmed/17652157
DOI: 10.1242/jcs.009506

Abstract

The mitotic spindle of vascular plants is assembled and maintained by processes that remain poorly explored at a molecular level. Here, we report that AtKRP125c, one of four kinesin-5 motor proteins in arabidopsis, decorates microtubules throughout the cell cycle and appears to function in both interphase and mitosis. In a temperature-sensitive mutant, interphase cortical microtubules are disorganized at the restrictive temperature and mitotic spindles are massively disrupted, consistent with a defect in the stabilization of anti-parallel microtubules in the spindle midzone, as previously described in kinesin-5 mutants from animals and yeast. AtKRP125c introduced into mammalian epithelial cells by transfection decorates microtubules throughout the cell cycle but is unable to complement the loss of the endogenous kinesin-5 motor (Eg5). These results are among the first reports of any motor with a major role in anastral spindle structure in plants and demonstrate that the conservation of kinesin-5 motor function throughout eukaryotes extends to vascular plants.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis thaliana AtKRP125 cortical microtubules Eg5 gamma-tubulin root morphology CROSS-LINKS Microtubules CULTURED TOBACCO CELLS mitotic spindle GAMMA-TUBULIN CORTICAL MICROTUBULES CYTOKINETIC APPARATUS STABLE EXPRESSION MAMMALIAN-CELLS SMALL-MOLECULE CARROT CELLS
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > kinesin-5 protein
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > microtubule
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > mitotic spindle
organism description > plant
CSHL Authors:
Communities: CSHL labs > Lukowitz lab
Depositing User: CSHL Librarian
Date: August 2007
Date Deposited: 02 Dec 2011 17:25
Last Modified: 21 Mar 2018 14:31
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22966

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