Developmentally regulated piRNA clusters implicate MILI in transposon control

Aravin, A. A., Sachidanandam, R., Girard, A., Fejes-Toth, K., Hannon, G. J. (May 2007) Developmentally regulated piRNA clusters implicate MILI in transposon control. Science, 316 (5825). pp. 744-7. ISSN 1095-9203 (Electronic)

URL: http://www.ncbi.nlm.nih.gov/pubmed/17446352
DOI: 10.1126/science.1142612

Abstract

Nearly half of the mammalian genome is composed of repeated sequences. In Drosophila, Piwi proteins exert control over transposons. However, mammalian Piwi proteins, MIWI and MILI, partner with Piwi-interacting RNAs (piRNAs) that are depleted of repeat sequences, which raises questions about a role for mammalian Piwi's in transposon control. A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. We also find evidence of an adaptive amplification loop in which MILI catalyzes the formation of piRNA 5' ends. Mili mutants derepress LINE-1 (L1) and intracisternal A particle and lose DNA methylation of L1 elements, demonstrating an evolutionarily conserved role for PIWI proteins in transposon suppression.

Item Type: Paper
Uncontrolled Keywords: 3' Untranslated Regions Animals Cluster Analysis Computational Biology DNA Methylation Genes, Intracisternal A-Particle Long Interspersed Nucleotide Elements Male Meiosis Mice Mutation Proteins/ metabolism RNA, Antisense genetics metabolism RNA, Untranslated genetics/metabolism Retroelements Reverse Transcriptase Polymerase Chain Reaction Short Interspersed Nucleotide Elements Spermatocytes cytology/ metabolism Spermatogenesis Suppression, Genetic
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Piwi protein
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL labs > Sachidanandam lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: 4 May 2007
Date Deposited: 02 Dec 2011 16:16
Last Modified: 22 Sep 2014 14:11
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22959

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving