Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons

Zhong, J., Zhang, T., Bloch, L. M. (February 2006) Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons. BMC Neuroscience, 7. p. 17. ISSN 14712202

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URL: https://www.ncbi.nlm.nih.gov/pubmed/16503994
DOI: 10.1186/1471-2202-7-17

Abstract

Background: Targeted transport of messenger RNA and local protein synthesis near the synapse are important for synaptic plasticity. In order to gain an overview of the composition of the dendritic mRNA pool, we dissected out stratum radiatum (dendritic lamina) from rat hippocampal CAI region and compared its mRNA content with that of stratum pyramidale (cell body layer) using a set of cDNA microarrays. RNAs that have over-representation in the dendritic fraction were annotated and sorted into function groups. Results: We have identified 154 dendritic mRNA candidates, which can be arranged into the categories of receptors and channels, signaling molecules, cytoskeleton and adhesion molecules, and factors that are involved in membrane trafficking, in protein synthesis, in posttranslational protein modification, and in protein degradation. Previously known dendritic mRNAs such as MAP2, calmodulin, and G protein gamma subunit were identified from our screening, as were mRNAs that encode proteins known to be important for synaptic plasticity and memory, such as spinophilin, Pumilio, eEFIA, and MHC class I molecules. Furthermore, mRNAs coding for ribosomal proteins were also found in dendrites. Conclusion: Our results suggest that neurons transport a variety of mRNAs to dendrites, not only those directly involved in modulating synaptic plasticity, but also others that play more common roles in cellular metabolism. © 2006 Zhong et al; licensee BioMed Central Ltd.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells

organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat

organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Zhang lab
Depositing User: CSHL Librarian
Date: February 2006
Date Deposited: 06 Dec 2011 18:54
Last Modified: 01 May 2018 16:05
PMCID: PMC1386695
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22953

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