Spine growth precedes synapse formation in the adult neocortex in vivo

Knott, G. W., Holtmaat, A. J. G. D., Wilbrecht, L., Welker, E., Svoboda, K. (August 2006) Spine growth precedes synapse formation in the adult neocortex in vivo. Nature Neuroscience, 9 (9). pp. 1117-1124. ISSN 10976256

URL: http://www.ncbi.nlm.nih.gov/pubmed/16892056
DOI: 10.1038/nn1747

Abstract

Dendritic spines appear and disappear in an experience-dependent manner. Although some new spines have been shown to contain synapses, little is known about the relationship between spine addition and synapse formation, the relative time course of these events, or whether they are coupled to de novo growth of axonal boutons. We imaged dendrites in barrel cortex of adult mice over 1 month, tracking gains and losses of spines. Using serial section electron microscopy, we analyzed the ultrastructure of spines and associated boutons. Spines reconstructed shortly after they appeared often lacked synapses, whereas spines that persisted for 4 d or more always had synapses. New spines had a large surface-to-volume ratio and preferentially contacted boutons with other synapses. In some instances, two new spines contacted the same axon. Our data show that spine growth precedes synapse formation and that new synapses form preferentially onto existing boutons. © 2006 Nature Publishing Group.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > axon
organs, tissues, organelles, cell types and functions > tissues types and functions > barrel cortex
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells

Investigative techniques and equipment > microscopy > electron microscopy
organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Depositing User: CSHL Librarian
Date: 6 August 2006
Date Deposited: 13 Dec 2011 15:42
Last Modified: 01 Mar 2013 15:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22840

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