Argonaute slicing is required for heterochromatic silencing and spreading

Irvine, D. V., Zaratiegui, Mi., Tolia, N. H., Goto, D. B., Chitwood, D. H., Vaughn, M. W., Joshua-Tor, L., Martienssen, R. A. (August 2006) Argonaute slicing is required for heterochromatic silencing and spreading. Science, 313 (5790). pp. 1134-7. ISSN 1095-9203 (Electronic)

URL: http://www.ncbi.nlm.nih.gov/pubmed/16931764
DOI: 10.1126/science.1128813

Abstract

Small interfering RNA (siRNA) guides dimethylation of histone H3 lysine-9 (H3K9me2) via the Argonaute and RNA-dependent RNA polymerase complexes, as well as base-pairing with either RNA or DNA. We show that Argonaute requires the conserved aspartate-aspartate-histidine motif for heterochromatic silencing and for ribonuclease H-like cleavage (slicing) of target messages complementary to siRNA. In the fission yeast Schizosaccharomyces pombe, heterochromatic repeats are transcribed by polymerase II. We show that H3K9me2 spreads into silent reporter genes when they are embedded within these transcripts and that spreading requires read-through transcription, as well as slicing by Argonaute. Thus, siRNA guides histone modification by basepairing interactions with RNA.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Motifs Base Pairing Genes Reporter Heterochromatin genetics metabolism Histones metabolism RNA Interference RNA Fungal metabolism RNA Messenger metabolism RNA Small Interfering metabolism Recombinant Fusion Proteins/metabolism Schizosaccharomyces genetics/metabolism Schizosaccharomyces pombe Proteins chemistry genetics/ metabolism Transcription Genetic
Subjects: organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
CSHL Authors:
Communities: CSHL labs > Joshua-Tor lab
CSHL labs > Martienssen lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: 25 August 2006
Date Deposited: 14 Dec 2011 15:35
Last Modified: 19 Sep 2014 18:44
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22827

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