Crystal structure of the HP1-EMSY complex reveals an unusual mode of HP1 binding

Huang, Y., Myers, M. P., Xu, R. M. (2006) Crystal structure of the HP1-EMSY complex reveals an unusual mode of HP1 binding. Structure, 14 (4). pp. 703-12. ISSN 0969-2126 (Print)

DOI: 10.1016/j.str.2006.01.007


Heterochromatin protein-1 (HP1) plays an essential role in both the assembly of higher-order chromatin structure and epigenetic inheritance. The C-terminal chromo shadow domain (CSD) of HP1 is responsible for homodimerization and interaction with a number of chromatin-associated nonhistone proteins, including EMSY, which is a BRCA2-interacting protein that has been implicated in the development of breast and ovarian cancer. We have determined the crystal structure of the HP1beta CSD in complex with the N-terminal domain of EMSY at 1.8 A resolution. Surprisingly, the structure reveals that EMSY is bound by two HP1 CSD homodimers, and the binding sequences differ from the consensus HP1 binding motif PXVXL. This structural information expands our understanding of HP1 binding specificity and provides insights into interactions between HP1 homodimers that are likely to be important for heterochromatin formation.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence BRCA2 Protein chemistry Binding Sites Breast Neoplasms metabolism Chromatography Gel Chromosomal Proteins Non-Histone chemistry metabolism Crystallography X-Ray DNA Complementary metabolism Dimerization Female Glutathione Transferase metabolism Heterochromatin metabolism Humans Mass Spectrometry Models Molecular Molecular Conformation Molecular Sequence Data Mutagenesis Mutagenesis Site-Directed Mutation Nucleosomes metabolism Ovarian Neoplasms metabolism Protein Binding Protein Conformation Protein Structure Tertiary Stereoisomerism Time Factors X-Ray Diffraction
Subjects: Investigative techniques and equipment > X-Ray Diffraction
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
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Depositing User: CSHL Librarian
Date Deposited: 14 Dec 2011 15:10
Last Modified: 14 Dec 2011 15:10

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