Sensory-evoked intrinsic optical signals in the olfactory bulb are coupled to glutamate release and uptake

Gurden, H., Uchida, N., Mainen, Z. F. (2006) Sensory-evoked intrinsic optical signals in the olfactory bulb are coupled to glutamate release and uptake. Neuron, 52 (2). pp. 335-45. ISSN 0896-6273 (Print)

URL: http://www.sciencedirect.com/science?_ob=ArticleUR...
DOI: 10.1016/j.neuron.2006.07.022

Abstract

Functional imaging signals arise from metabolic and hemodynamic activity, but how these processes are related to the synaptic and electrical activity of neurons is not well understood. To provide insight into this issue, we used in vivo imaging and simultaneous local pharmacology to study how sensory-evoked neural activity leads to intrinsic optical signals (IOS) in the well-defined circuitry of the olfactory glomerulus. Odor-evoked IOS were tightly coupled to release of glutamate and were strongly modulated by activation of presynaptic dopamine and GABA-B receptors. Surprisingly, IOS were independent of postsynaptic transmission through ionotropic or metabotropic glutamate receptors, but instead were inhibited when uptake by astrocytic glutamate transporters was blocked. These data suggest that presynaptic glutamate release and uptake by astrocytes form a critical pathway through which neural activity is linked to metabolic processing and hence to functional imaging signals.

Item Type: Paper
Uncontrolled Keywords: Animals Astrocytes drug effects metabolism secretion Cell Communication physiology Cerebrovascular Circulation physiology Dopamine metabolism Energy Metabolism physiology Excitatory Amino Acid Transporter metabolism Excitatory Amino Acid Transporter 2 antagonists & inhibitors metabolism Glutamic Acid metabolism Light Models Neurological Olfactory Bulb metabolism Optics Presynaptic Terminals metabolism Rats Rats Long-Evans Receptors, Dopamine/metabolism Receptors GABA-B metabolism Receptors Glutamate metabolism Synaptic Transmission physiology gamma-Aminobutyric Acid metabolism
Subjects: Investigative techniques and equipment > electrophysiology
Investigative techniques and equipment > imaging
organism description > animal behavior > odor recognition
organs, tissues, organelles, cell types and functions > tissues types and functions > olfactory bulb
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Depositing User: CSHL Librarian
Date Deposited: 15 Dec 2011 15:12
Last Modified: 15 Dec 2011 15:12
URI: http://repository.cshl.edu/id/eprint/22807

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