Lessons from Nature: microRNA-based shRNA libraries

Chang, K., Elledge, S. J., Hannon, G. J. (September 2006) Lessons from Nature: microRNA-based shRNA libraries. Nat Methods, 3 (9). pp. 707-14. ISSN 1548-7091 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/16929316
DOI: 10.1038/nmeth923

Abstract

Loss-of-function genetics has proven essential for interrogating the functions of genes and for probing their roles within the complex circuitry of biological pathways. In many systems, technologies allowing the use of such approaches were lacking before the discovery of RNA interference (RNAi). We have constructed first-generation short hairpin RNA (shRNA) libraries modeled after precursor microRNAs (miRNAs) and second-generation libraries modeled after primary miRNA transcripts (the Hannon-Elledge libraries). These libraries were arrayed, sequence-verified, and cover a substantial portion of all known and predicted genes in the human and mouse genomes. Comparison of first- and second-generation libraries indicates that RNAi triggers that enter the RNAi pathway through a more natural route yield more effective silencing. These large-scale resources are functionally versatile, as they can be used in transient and stable studies, and for constitutive or inducible silencing. Library cassettes can be easily shuttled into vectors that contain different promoters and/or that provide different modes of viral delivery.

Item Type: Paper
Uncontrolled Keywords: Animals Gene Library Humans MicroRNAs genetics Plasmids genetics RNA Interference RNA, Small Interfering genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > loss of function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: CSHL Librarian
Date: September 2006
Date Deposited: 19 Dec 2011 18:13
Last Modified: 02 Dec 2016 15:50
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22770

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