Synaptic incorporation of AMPA receptors during LTP is controlled by a PKC phosphorylation site on GluR1

Boehm, J., Kang, M-G., Johnson, R. C., Esteban, J. A., Huganir, R. L., Malinow, R. (July 2006) Synaptic incorporation of AMPA receptors during LTP is controlled by a PKC phosphorylation site on GluR1. Neuron, 51 (2). pp. 213-25. ISSN 0896-6273 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/16846856
DOI: 10.1016/j.neuron.2006.06.013

Abstract

Incorporation of GluR1-containing AMPA receptors into synapses is essential to several forms of neural plasticity, including long-term potentiation (LTP). Numerous signaling pathways that trigger this process have been identified, but the direct modifications of GluR1 that control its incorporation into synapses are unclear. Here, we show that phosphorylation of GluR1 by PKC at a highly conserved serine 818 residue is increased during LTP and critical for LTP expression. GluR1 is phosphorylated by PKC at this site in vitro and in vivo. In addition, acute phosphorylation at GluR1 S818 by PKC, as well as a phosphomimetic mutation, promotes GluR1 synaptic incorporation. Conversely, preventing GluR1 S818 phosphorylation reduces LTP and blocks PKC-driven synaptic incorporation of GluR1. We conclude that the phosphorylation of GluR1 S818 by PKC is a critical event in the plasticity-driven synaptic incorporation of AMPA receptors.

Item Type: Paper
Uncontrolled Keywords: Animals Binding Sites genetics Cell Line Humans Long-Term Potentiation genetics Mutagenesis Site-Directed Phosphorylation Protein Kinase C genetics metabolism physiology Rats Receptors AMPA genetics metabolism physiology Synapses genetics metabolism physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neural plasticity
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: CSHL Librarian
Date: 20 July 2006
Date Deposited: 21 Dec 2011 14:41
Last Modified: 12 Apr 2018 16:08
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22755

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving