Depolarizing GABAergic conductances regulate the balance of excitation to inhibition in the developing retinotectal circuit in vivo

Akerman, C. J., Cline, H. T. (2006) Depolarizing GABAergic conductances regulate the balance of excitation to inhibition in the developing retinotectal circuit in vivo. J Neurosci, 26 (19). pp. 5117-30. ISSN 1529-2401 (Electronic)

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Depolarizing-GABAergic-conductances-regulate-the-balance-of-excitation-to-inhibition-in-the-developing-retinotectal-circuit-in-vivo_2006_Journal-of-Neuroscience.pdf - Published Version

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URL: http://www.jneurosci.org/cgi/content/full/26/19/51...
DOI: 10.1523/jneurosci.0319-06.2006

Abstract

Neurotransmission during development regulates synaptic maturation in neural circuits, but the contribution of different neurotransmitter systems is unclear. We investigated the role of GABAA receptor-mediated Cl- conductances in the development of synaptic responses in the Xenopus visual system. Intracellular Cl- concentration ([Cl-]i) was found to be high in immature tectal neurons and then falls over a period of several weeks. GABAergic synapses are present at early stages of tectal development and, when activated by optic nerve stimulation or visual stimuli, induce sustained depolarizing Cl- conductances that facilitate retinotectal transmission by NMDA receptors. To test whether depolarizing GABAergic inputs cooperate with NMDA receptors during activity-dependent maturation of glutamatergic synapses, we prematurely reduced [Cl-]i in tectal neurons in vivo by expressing the Cl- transporter KCC2. This blocked the normal developmental increase in AMPA receptor-mediated retinotectal transmission and increased GABAergic synaptic input to tectal neurons. Therefore, depolarizing GABAergic transmission plays a pivotal role in the maturation of excitatory transmission and controls the balance of excitation and inhibition in the developing retinotectal circuit.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Membrane physiology Chloride Channels physiology Electric Conductivity Excitatory Postsynaptic Potentials physiology Larva Membrane Potentials physiology Neural Inhibition physiology Synaptic Transmission physiology Visual Pathways embryology physiology Xenopus laevis physiology gamma-Aminobutyric Acid metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > small molecules > GABAergic
Investigative techniques and equipment > electrophysiology
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
organism description > animal behavior > visual
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: CSHL Librarian
Date Deposited: 20 Dec 2011 17:06
Last Modified: 31 Jan 2012 21:03
URI: http://repository.cshl.edu/id/eprint/22747

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