Laminar and columnar organization of ascending excitatory projections to layer 2/3 pyramidal neurons in rat barrel cortex

Shepherd, G. M., Svoboda, K. (June 2005) Laminar and columnar organization of ascending excitatory projections to layer 2/3 pyramidal neurons in rat barrel cortex. J Neurosci, 25 (24). pp. 5670-9. ISSN 0270-6474

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URL: https://www.ncbi.nlm.nih.gov/pubmed/15958733
DOI: 10.1523/jneurosci.1173-05.2005

Abstract

Excitatory synaptic projections to layer 2/3 (L2/3) pyramidal neurons in brain slices from the rat barrel cortex were measured using quantitative laser-scanning photostimulation (LSPS) mapping. In the barrel cortex, cytoarchitectonic "barrels" and "septa" in L4 define a stereotypical array of landmarks, allowing alignment and averaging of LSPS maps from multiple cells in different slices. We distinguished inputs to L2 and L3 neurons above barrels and septa. Average input maps revealed that barrel-related ascending projections (L4-->2/3barrel) interdigitated with a novel septum-related projection (L5A-->2septum). We also explored the functional organization of these projections by comparing the input maps of multiple cells in individual slices. L2/3 cells sharing the same barrel-related column showed strong correlations in their input maps, independent of their precise locations within the column; otherwise, correlations fell rapidly as a function of intersomatic separation. Our data indicate that barrel-related and septum-related columns are associated with distinct functional circuits. These projections are likely to mediate parallel processing of somatosensory signals within the barrel cortex, with L4-->2/3barrel and L5A-->2septum representing the intracortical continuations of, respectively, the subcortical lemniscal and paralemniscal systems conveying somatosensory information to the barrel cortex.

Item Type: Paper
Uncontrolled Keywords: Animals Electrophysiology methods Evoked Potentials Somatosensory drug effects/physiology Glutamates Indoles Photic Stimulation Pyramidal Cells physiology Rats Rats Sprague-Dawley Sprague Dawley Somatosensory Cortex physiology Synapses physiology Video Recording
Subjects: bioinformatics > genomics and proteomics > annotation > map annotation
Investigative techniques and equipment > optical devices > lasers > quantitative laser scanning photostimulation
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: CSHL Librarian
Date: 15 June 2005
Date Deposited: 05 Jan 2012 17:43
Last Modified: 11 Sep 2018 20:35
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22700

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