Maize rough sheath2 and its Arabidopsis orthologue ASYMMETRIC LEAVES1 interact with HIRA, a predicted histone chaperone, to maintain knox gene silencing and determinacy during organogenesis

Phelps-Durr, T. L., Thomas, J., Vahab, Phil, Timmermans, M.C.P. (November 2005) Maize rough sheath2 and its Arabidopsis orthologue ASYMMETRIC LEAVES1 interact with HIRA, a predicted histone chaperone, to maintain knox gene silencing and determinacy during organogenesis. Plant Cell, 17 (11). pp. 2886-98. ISSN 1040-4651 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/16243907
DOI: 10.1105/tpc.105.035477

Abstract

Plant shoots are characterized by indeterminate growth resulting from the action of a population of stem cells in the shoot apical meristem (SAM). Indeterminacy within the SAM is specified in part by the class I knox homeobox genes. The myb domain proteins rough sheath2 (RS2) and ASYMMETRIC LEAVES1 (AS1) from maize (Zea mays) and Arabidopsis thaliana, respectively, are required to establish determinacy during leaf development. These proteins are part of a cellular memory system that in response to a stem cell-derived signal keeps knox genes in an off state during organogenesis. Here, we show that RS2/AS1 can form conserved protein complexes through interaction with the DNA binding factor ASYMMETRIC LEAVES2, a predicted RNA binding protein (RIK, for RS2-Interacting KH protein), and a homologue of the chromatin-remodeling protein HIRA. Partial loss of HIRA function in Arabidopsis results in developmental defects comparable to those of as1 and causes reactivation of knox genes in developing leaves, demonstrating a direct role for HIRA in knox gene repression and the establishment of determinacy during leaf formation. Our data suggest that RS2/AS1 and HIRA mediate the epigenetic silencing of knox genes, possibly by modulating chromatin structure. Components of this process are conserved in animals, suggesting the possibility that a similar epigenetic mechanism maintains determinacy during both plant and animal development.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis embryology genetics metabolism Arabidopsis Proteins genetics metabolism Cell Cycle Proteins genetics metabolism Evolution Molecular Gene Expression Regulation Plant physiology Gene Silencing physiology Genes, MYB genetics Histones metabolism Homeodomain Proteins genetics metabolism Meristem embryology genetics metabolism Molecular Chaperones genetics/metabolism Molecular Sequence Data Phylogeny Plant Leaves embryology/genetics/metabolism Plant Proteins genetics/ metabolism Plant Shoots embryology/genetics/metabolism Repressor Proteins genetics/ metabolism Seeds genetics metabolism Sequence Homology, Amino Acid Stem Cells cytology metabolism Transcription Factors genetics/ metabolism Zea mays embryology genetics metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > chaperones
CSHL Authors:
Communities: CSHL labs > Timmermans lab
Depositing User: CSHL Librarian
Date: November 2005
Date Deposited: 09 Jan 2012 14:42
Last Modified: 17 Feb 2017 16:37
PMCID: PMC1276017
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22674

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