Transgenic mice overexpressing nNOS in the adult nervous system

Packer, M. A., Hemish, Jill, Mignone, J. L., John, S., Pugach, I., Enikolopov, Grigori (September 2005) Transgenic mice overexpressing nNOS in the adult nervous system. Cell Mol Biol (Noisy-le-grand), 51 (3). pp. 269-77. ISSN 1165-158X (Electronic)

URL: https://www.ncbi.nlm.nih.gov/pubmed/16191394

Abstract

Nitric oxide (NO) has a profound role in the generation, differentiation, survival, and physiology of neurons. We have created a novel transgenic model to study the action of NO in the adult brain, in which the neuronal isoform of NO synthase (nNOS) is expressed under control of the promoter of the calcium-calmodulin multifunctional kinase IIalpha (CaMKIIalpha) gene. We show that the transgenic nNOS RNA and protein are expressed in the cortex, the hippocampus and the striatum of the transgenic mice. We also show that expression of several genes involved in the protection of neurons from oxidative stress and cell death is not affected in neurons of the transgenic mice. Furthermore, generation of new cells is depressed in the neurogenic brain areas in transgenics. In addition, we analyze gene expression in the hippocampus of the transgenic animals using microarray RNA profiling and Q-PCR. Our experiments describe specific changes in cell division and gene activity in the CaMKII-nNOS transgenic model and demonstrate its utility for studying the action of NO in the adult brain.

Item Type: Paper
Uncontrolled Keywords: Animals Apoptosis genetics physiology nNOS Ca(2+)-Calmodulin Dependent Protein Kinase genetics metabolism Cell Division genetics Cerebral Cortex metabolism Corpus Striatum metabolism Gene Expression Profiling Gene Expression Regulation Enzymologic Hippocampus metabolism Mice Mice Transgenic Microscopy Confocal Nervous System metabolism Neurons physiology Nitric Oxide physiology Nitric Oxide Synthase Type I biosynthesis genetics Oligonucleotide Array Sequence Analysis Oxidative Stress genetics Polymerase Chain Reaction Promoter Regions (Genetics) RNA biosynthesis genetics Transgenes
Subjects: organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: CSHL Librarian
Date: 5 September 2005
Date Deposited: 06 Jan 2012 21:22
Last Modified: 01 Mar 2017 16:02
URI: http://repository.cshl.edu/id/eprint/22670

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