Genomic changes in synthetic Arabidopsis polyploids

Madlung, A., Tyagi, A. P., Watson, B., Jiang, H. M., Kagochi, T., Doerge, R. W., Martienssen, R. A., Comai, L. (January 2005) Genomic changes in synthetic Arabidopsis polyploids. Plant Journal, 41 (2). pp. 221-230. ISSN 0960-7412

URL: http://www.ncbi.nlm.nih.gov/pubmed/15634199
DOI: 10.1111/j.1365-313X.2004.02297.x

Abstract

Polyploids are common and arise frequently by genome duplication (autopolyploids) or interspecific hybridization (allopolyploids). Neoallopolyploids display sterility, lethality, phenotypic instability, gene silencing and epigenetic changes. Little is known about the molecular basis of these phenomena, and how much genomic remodeling happens upon allopolyploidization. Extensive genomic remodeling has been documented in wheat, but little remodeling occurs in cotton. Newly synthesized Arabidopsis allopolyploids, which display phenotypic instability and low fertility, displayed several, possibly related mechanisms that can remodel genomes. We detected transcriptional activity of several transposons although their transposition was limited. One represents a new family of conditionally active En-Spm-like transposons of Arabidopsis thaliana, which underwent remodeling of CG methylation upon allopolyploidization. A random amplified fragment length polymorphism survey suggested remodeling at few, specific loci. Meiotic analyses revealed the appearance of chromosomal fragments in a substantial fraction of anther meiocytes. In several individuals produced by hybrids between the synthetic and a natural allopolyploid pollen viability inversely correlated with meiotic instability. Activity of selected DNA transposons and the possibly related chromosomal breaks could cause changes by inducing translocations and rearrangements.

Item Type: Paper
Uncontrolled Keywords: allopolyploidy hybrid genome transposon epigenetics meiosis DNA methylation gene expression wheat chrosomes sequences allotetraploids alloploidy brassiciceae transposons elimination
Subjects: bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: CSHL Librarian
Date: January 2005
Date Deposited: 10 Jan 2012 16:14
Last Modified: 10 Apr 2014 14:09
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22642

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