The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators

Lee, D. Y., Ezhkova, E., Li, B., Pattenden, S. G., Tansey, W. P., Workman, J. L. (November 2005) The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators. Cell, 123 (3). pp. 423-436. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/16269334
DOI: 10.1016/j.cell.2005.08.015

Abstract

Promoter recruitment of the Saccharomyces cerevisiae SAGA histone acetyltransferase complex is required for RNA polymerase II-dependent transcription of several genes. SAGA is targeted to promoters through interactions with sequence-specific DNA binding transcriptional activators and facilitates preinitiation-complex assembly and transcription. Here, we show that the 19S proteasome regulatory particle (19S RP) alters SAGA to stimulate its interaction with transcriptional activators. The ATPase components of the 19S RP are required for stimulation of SAGA/activator interactions and enhance SAGA recruitment to promoters. Proteasomal ATPases genetically interact with SAGA, and their inhibition reduces global histone H3 acetylation levels and SAGA recruitment to target promoters in vivo. These results indicate that the 19S RIP modulates SAGA complex using its ATPase components, thereby facilitating subsequent transcription events at promoters.

Item Type: Paper
Uncontrolled Keywords: histone acetyltransferase complex TATA binding protein saccharomyces serviciae in-vivo SWI/SNF complex active genes acetylation recruitment H2B H3
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
CSHL Authors:
Communities: CSHL labs > Tansey lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: November 2005
Date Deposited: 09 Jan 2012 19:14
Last Modified: 19 Sep 2014 15:47
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22628

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