A novel cell-to-cell trafficking assay indicates that the KNOX homeodomain is necessary and sufficient for intercellular protein and mRNA trafficking

Kim, J. Y., Rim, Y., Wang, L., Jackson, D. P. (April 2005) A novel cell-to-cell trafficking assay indicates that the KNOX homeodomain is necessary and sufficient for intercellular protein and mRNA trafficking. Genes & Development, 19 (7). pp. 788-793. ISSN 0890-9369

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URL: https://www.ncbi.nlm.nih.gov/pubmed/15805469
DOI: 10.1101/gad.332805

Abstract

Cell-to-cell trafficking of regulatory proteins is a novel mechanism for communication during cell fate specification in plants. Although several developmental proteins traffic cell-to-cell, no signals that are both necessary and sufficient for this function in developmental proteins have been described. We developed a novel trafficking assay using trichome rescue in Arabidopsis. Fusion to KNOTTED1 (KN1) conferred gain-of-trafficking function to the cell-autonomous GLABROUS1 (GL1) protein. We show that the KNOX homeodomain (HD) is necessary and sufficient for intercellular trafficking, identifying a novel function for the HD as the minimal sequence required for trafficking of KN1 and its associated mRNA.

Item Type: Paper
Uncontrolled Keywords: KNOX homeodomain trichome trafficking signal Glabrous 1 knotted1 tobacco mosaic virus movement protein shoot meristem homeobox gene selective trafficking Arabidopsis expression plants maize leaf
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: CSHL Librarian
Date: April 2005
Date Deposited: 12 Jan 2012 17:12
Last Modified: 11 Jan 2017 21:27
PMCID: PMC1074316
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22622

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