Regulation of cpg15 expression during single whisker experience in the barrel cortex of adult mice

Harwell, C., Burbach, B., Svoboda, K., Nedivi, E. (October 2005) Regulation of cpg15 expression during single whisker experience in the barrel cortex of adult mice. Journal of Neurobiology, 65 (1). pp. 85-96. ISSN 0022-3034

URL: https://www.ncbi.nlm.nih.gov/pubmed/16010668
DOI: 10.1002/neu.20176

Abstract

Regulation of gene transcription by neuronal activity is thought to be key to the translation of sensory experience into long-term changes in synaptic structure and function. Here we show that cpgI5, a gene encoding an extracellular signaling molecule that promotes dendritic and axonal growth and synaptic maturation, is regulated in the somatosensory cortex by sensory experience capable of inducing cortical plasticity. Using in situ hybridization, we monitored cpgI5 expression in 4-week-old mouse barrel cortex after trimming all whiskers except D1. We found that cpgI5 expression is depressed in the deprived barrels and enhanced in the barrel column corresponding to the spared D1 whisker. Changes in cpgI5 mRNA levels first appear in layer IV, peak 12 h after deprivation, and then decline rapidly. In layers II/III, changes in cpgI5 expression appear later, peak at 24 h, and persist for days. Induction of cpgI5 expression is significantly diminished in adolescent as well as adult CREB knockout mice. cpgI5's spatio-temporal expression pattern and its regulation by CREB are consistent with a role in experience-dependent plasticity of cortical circuits. Our results suggest that local structural and/or synaptic changes may be a mechanism by which the adult cortex can adapt to peripheral manipulations. (c) 2005 Wiley Periodicals, Inc.

Item Type: Paper
Uncontrolled Keywords: barrel cortex cpg15 deprivation in-situ hybridization experience-dependent plasticity dependent long term potentiation in-vivo targeted mutation up regulation up-regulation C-FOS GENE alpha CREB mechanisms
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > axon
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells

organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: CSHL Librarian
Date: October 2005
Date Deposited: 13 Jan 2012 15:33
Last Modified: 03 May 2018 20:11
PMCID: PMC3062911
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22587

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