Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton

Zito, K., Knott, G., Shepherd, G. M., Shenolikar, S., Svoboda, K. (October 2004) Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton. Neuron, 44 (2). pp. 321-34. ISSN 0896-6273 (Print)

URL: http://www.sciencedirect.com/science?_ob=ArticleUR...
DOI: 10.1016/j.neuron.2004.09.022

Abstract

We explored the relationship between regulation of the spine actin cytoskeleton, spine morphogenesis, and synapse formation by manipulating expression of the actin binding protein NrbI and its deletion mutants. In pyramidal neurons of cultured rat hippocampal slices, NrbI is concentrated in dendritic spines by binding to the actin cytoskeleton. Expression of one NrbI deletion mutant, containing the actin binding domain, dramatically increased the density and length of dendritic spines with synapses. This hyperspinogenesis was accompanied by enhanced actin polymerization and spine motility. Synaptic strengths were reduced to compensate for extra synapses, keeping total synaptic input per neuron constant. Our data support a model in which synapse formation is promoted by actin-powered motility.

Item Type: Paper
Uncontrolled Keywords: Actins physiology Amino Acid Sequence Animals Cytoskeleton physiology Dendrites physiology ultrastructure Microfilament Proteins genetics metabolism Microscopy Electron Models Neurological Molecular Sequence Data Mutation Nerve Tissue Proteins genetics/metabolism Neuronal Plasticity Patch-Clamp Techniques Pyramidal Cells cytology Rats Sequence Homology Synapses physiology ultrastructure Transfection
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > actin
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
CSHL Authors:
Depositing User: CSHL Librarian
Date: 14 October 2004
Date Deposited: 17 Jan 2012 15:07
Last Modified: 17 Jan 2012 15:07
URI: http://repository.cshl.edu/id/eprint/22527

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