Correlation maps allow neuronal electrical properties to be predicted from single-cell gene expression profiles in rat neocortex

Toledo-Rodriguez, M., Blumenfeld, B., Wu, C. Z., Luo, J. Y., Attali, B., Goodman, P., Markram, H. (December 2004) Correlation maps allow neuronal electrical properties to be predicted from single-cell gene expression profiles in rat neocortex. Cerebral Cortex, 14 (12). pp. 1310-1327. ISSN 1047-3211

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DOI: 10.1093/cercor/bhh092

Abstract

The computational power of the neocortex arises from interactions of multiple neurons, which display a wide range of electrical properties. The gene expression profiles underlying this phenotypic diversity are unknown. To explore this relationship, we combined whole-cell electrical recordings with single-cell multiplex RT-PCR of rat (p13-16) neocortical neurons to obtain cDNA libraries of 26 ion channels (including voltage activated potassium channels, Kv1.1/2/4/6, Kvbeta1/2, Kv2.1/2, Kv3.1/2/3/4, Kv4.2/3; sodium/potassium permeable hyperpolarization activated channels, HCN1/2/3/4; the calcium activated potassium channel, SK2; voltage activated calcium channels, Caalpha1A/B/G/I, Cabeta1/3/4), three calcium binding proteins (calbindin, parvalbumin and calretinin) and GAPOH. We found a previously unreported clustering of ion channel genes around the three calcium-binding proteins. We further determined that cells similar in their expression patterns were also similar in their electrical properties. Subsequent regression modeling with statistical resampling yielded a set of coefficients that reliably predicted electrical properties from the expression profile of individual neurons. This is the first report of a consistent relationship between the co-expression of a large profile of ion channel and calcium binding protein genes and the electrical phenotype of individual neocortical neurons.

Item Type: Paper
Uncontrolled Keywords: cortex electrophysiology ion channel neuron single-cell RT-PCR GATED K+ CHANNELS gated K+ channels NEOSTRIATAL CHOLINERGIC INTERNEURONS neostriatal cholinergic interneurons POLYMERASE polymerase CHAIN-REACTION chain reaction PYRAMIDAL NEURONS pyramidal neurons SYNAPTIC CONNECTIONS synaptic connections BETA-SUBUNIT beta-subunit DIFFERENTIAL DISTRIBUTION differential distribution CALCIUM-CHANNELS calcium channels CA2+ CHANNELS ION CHANNELS ion channels
Subjects: Investigative techniques and equipment > electrophysiology
CSHL Authors:
Depositing User: CSHL Librarian
Date: December 2004
Date Deposited: 17 Jan 2012 16:54
Last Modified: 17 Jan 2012 16:54
URI: http://repository.cshl.edu/id/eprint/22506

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