RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age

Silva, J., Chang, K., Hannon, G. J., Rivas, F. V. (November 2004) RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age. Oncogene, 23 (51). pp. 8401-9. ISSN 0950-9232 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/15517022
DOI: 10.1038/sj.onc.1208176

Abstract

Sequencing of complete genomes has provided researchers with a wealth of information to study genome organization, genetic instability, and polymorphisms, as well as a knowledge of all potentially expressed genes. The identification of all genes encoded in the human genome opens the door for large-scale systematic gene silencing using small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs). With the recent development of siRNA and shRNA expression libraries, the application of RNAi technology to assign function to cancer genes and to delineate molecular pathways in which these genes affect in normal and transformed cells, will contribute significantly to the knowledge necessary to develop new and also improve existing cancer therapy.

Item Type: Paper
Uncontrolled Keywords: Animals Gene Library Genomics Humans RNA Interference physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: CSHL Librarian
Date: 1 November 2004
Date Deposited: 26 Jan 2012 15:23
Last Modified: 02 Dec 2016 15:58
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22481

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