The GAOLAOZHUANGREN1 gene encodes a putative glycosyltransferase that is critical for normal development and carbohydrate metabolism

Shao, M. H., Zheng, H. Q., Hu, Y., Liu, D. H., Jang, J. C., Ma, H., Huang, H. (October 2004) The GAOLAOZHUANGREN1 gene encodes a putative glycosyltransferase that is critical for normal development and carbohydrate metabolism. Plant and Cell Physiology, 45 (10). pp. 1453-1460. ISSN 0032-0781

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DOI: 10.1093/pcp/pch168

Abstract

Glycosyltransferases are enzymes that catalyze the attachment of a sugar molecule to specific acceptor molecules. These enzymes have been shown to play important roles in a number of biological processes. Whereas a large number of putative glycosyltransferase genes have been identified by genomic sequencing, the functions of most of these genes are unknown. Here we report the characterization of an Arabidopsis mutant, designated gaolaozhuangren1 (glz1), which is allelic to parvus characterized recently. The glz1 mutant exhibited a reduced plant stature, reduced size of organs in the shoot and dark-green leaves, indicating an important role of GLZ1 gene in normal development. The earliest GLZ1 expression appears at the shoot apical region of 4-d-old seedlings, which coincides with the onset of the glz1 morphological phenotypes. GLZ1 is expressed in a tissue-specific and developmentally regulated manner, predominantly in the stem and silique, and moderately in the flower. GLZ1 expression is strong in the midrib of rosette and cauline leaves; however, its expression was not detectable in the midrib of the cotyledon. Further analyses revealed that carbohydrate composition and distribution were aberrant in the glz1 mutant. These, together with the GLZ1 expression pattern, suggest a requirement for the GLZ1 function in normal sink-source transition during plant development.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis assimilate distribution development glycosyltransferases ARABIDOPSIS-THALIANA Arabidopsis Thaliana UDP-GLUCOSE UDP-glucose LEAVES GLUCOSYLTRANSFERASE Glucosyltransferase EXPRESSION expression PROTEIN protein CARBON carbon CLASSIFICATION classification ENZYMES enzymes CLONING cloning
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
CSHL Authors:
Depositing User: CSHL Librarian
Date: October 2004
Date Deposited: 26 Jan 2012 14:54
Last Modified: 26 Jan 2012 14:54
URI: http://repository.cshl.edu/id/eprint/22477

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