New synaptic bouton formation is disrupted by misregulation of microtubule stability in aPKC mutants

Ruiz-Canada, C., Ashley, J., Moeckel-Cole, S., Drier, E., Yin, J., Budnik, V. (May 2004) New synaptic bouton formation is disrupted by misregulation of microtubule stability in aPKC mutants. Neuron, 42 (4). pp. 567-580. ISSN 0896-6273

URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

The Baz/Par-3-Par-6-aPKC complex is an evolutionarily conserved cassette critical for the development of polarity in epithelial cells, neuroblasts, and oocytes. aPKC is also implicated in long-term synaptic plasticity in mammals and the persistence of memory in flies, suggesting a synaptic function for this cassette. Here we show that at Drosophila glutamatergic synapses, aPKC controls the formation and structure of synapses by regulating microtubule (MT) dynamics. At the presynapse, aPKC regulates the stability of MTs by promoting the association of the MAP1B-related protein Futsch to MTs. At the postsynapse, aPKC regulates the synaptic cytoskeleton by controlling the extent of Actin-rich and MT-rich areas. In addition, we show that Baz and Par-6 are also expressed at synapses and that their synaptic localization depends on aPKC activity. Our findings establish a novel role for this complex during synapse development and provide a cellular context for understanding the role of aPKC in synaptic plasticity and memory.

Item Type: Paper
Uncontrolled Keywords: PROTEIN-KINASE-C protein kinase-C CELL-POLARITY cell polarity ALPHA-ACTININ alpha actinin GROWTH CONES growth cones DISCS-LARGE discs large DROSOPHILA drosophila NEUROBLASTS neuroblasts COMPLEX complex BAZOOKA bazooka BINDING binding
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell polarity
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cell

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells

organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neural plasticity
CSHL Authors:
Depositing User: CSHL Librarian
Date: May 2004
Date Deposited: 26 Jan 2012 21:42
Last Modified: 26 Jan 2012 21:42
URI: http://repository.cshl.edu/id/eprint/22468

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