Spatially restricted microRNA directs leaf polarity through ARGONAUTE1

Kidner, C. A., Martienssen, R. A. (March 2004) Spatially restricted microRNA directs leaf polarity through ARGONAUTE1. Nature, 428 (6978). pp. 81-4. ISSN 1476-4687 (Electronic)

URL: http://www.ncbi.nlm.nih.gov/pubmed/14999284
DOI: 10.1038/nature02366

Abstract

Gene regulation by RNA interference requires the functions of the PAZ domain protein Argonaute. In plants, mutations in ARGONAUTE1 (AGO1) are associated with distinctive developmental defects that suggest a role for microRNA (miRNA) in organ polarity. Potential targets of miRNA regulation are the homeodomain/leucine zipper genes PHABULOSA (PHB) and PHAVOLUTA (PHV). These genes are expressed in a polar fashion in leaf primordia and are required for adaxial cell fate. Here we show that a 21-nucleotide miRNA that directs cleavage of PHB/PHV messenger RNA accumulates first in the embryonic meristem, and then in the abaxial domain of the developing leaf. miRNA distribution is disrupted by mutations in AGO1, indicating that AGO1 affects the regulation of miRNA. In addition, interactions between homeodomain/leucine zipper genes and an allelic series of ago1 indicate that miRNA acts as a signal to specify leaf polarity.

Item Type: Paper
Uncontrolled Keywords: Alleles Arabidopsis embryology genetics metabolism Arabidopsis Proteins genetics metabolism Body Patterning Gene Expression Regulation Plant Genes Plant genetics Genes Reporter Meristem embryology genetics metabolism MicroRNAs genetics metabolism Mutation genetics Plant Leaves embryology genetics metabolism RNA, Messenger genetics metabolism RNA Plant genetics metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

organism description > plant behavior > plant orientation > plant polarity
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: CSHL Librarian
Date: 4 March 2004
Date Deposited: 01 Feb 2012 16:47
Last Modified: 10 Apr 2014 15:14
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22411

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