microRNA-mediated repression of rolled leaf1 specifies maize leaf polarity

Juarez, M. T., Kui, J. S., Thomas, J., Heller, B. A., Timmermans, M. C. (March 2004) microRNA-mediated repression of rolled leaf1 specifies maize leaf polarity. Nature, 428 (6978). pp. 84-8. ISSN 1476-4687 (Electronic)

URL: http://www.ncbi.nlm.nih.gov/pubmed/14999285
DOI: 10.1038/nature02363

Abstract

In both animals and plants, many developmentally important regulatory genes have complementary microRNAs (miRNAs), which suggests that these miRNAs constitute a class of developmental signalling molecules. Leaves of higher plants exhibit a varying degree of asymmetry along the adaxial/abaxial (upper/lower) axis. This asymmetry is specified through the polarized expression of class III homeodomain/leucine zipper (HD-ZIPIII) genes. In Arabidopsis, three such genes, PHABULOSA (PHB), PHAVOLUTA (PHV) and REVOLUTA (REV), are expressed throughout the incipient leaf, but become adaxially localized after primordium emergence. Downregulation of the HD-ZIPIII genes allows expression of the KANADI and YABBY genes, which specify abaxial fate. PHB, PHV and REV transcripts contain a complementary site for miRNA165 and miRNA166, which can direct their cleavage in vitro. Here we show that miRNA166 constitutes a highly conserved polarizing signal whose expression pattern spatially defines the expression domain of the maize hd-zipIII family member rolled leaf1 (rld1). Moreover, the progressively expanding expression pattern of miRNA166 during leaf development and its accumulation in phloem suggests that miRNA166 may form a movable signal that emanates from a signalling centre below the incipient leaf.

Item Type: Paper
Uncontrolled Keywords: Alleles Amino Acid Sequence Base Sequence Body Patterning Down-Regulation Gene Expression Regulation, Plant Genes Plant genetics MicroRNAs genetics metabolism Molecular Sequence Data Phenotype Plant Leaves embryology genetics Plant Proteins chemistry genetics metabolism RNA Interference RNA Plant genetics metabolism Zea mays embryology genetics
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
CSHL Authors:
Communities: CSHL labs > Timmermans lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: 4 March 2004
Date Deposited: 01 Feb 2012 15:44
Last Modified: 19 Sep 2014 15:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22403

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