Functional characterization of the HD-ZIP IV transcription factor OCL1 from maize

Depge-Fargeix, N., Javelle, M., Chambrier, P., Frangne, N., Gerentes, D., Perez, P., Rogowsky, P. M., Vernoud, V. (2011) Functional characterization of the HD-ZIP IV transcription factor OCL1 from maize. Journal of Experimental Botany, 62 (1). pp. 293-305.

URL: http://www.ncbi.nlm.nih.gov/pubmed/20819789
DOI: 10.1093/jxb/erq267

Abstract

OCL1 (OUTER CELL LAYER1) encodes a maize HD-ZIP class IV transcription factor (TF) characterized by the presence of a homeo DNA-binding domain (HD), a dimerization leucine zipper domain (ZIP), and a steroidogenic acute regulatory protein (StAR)-related lipid transfer domain (START) involved in lipid transport in animals but the function of which is still unknown in plants. By combining yeast and plant trans-activation assays, the transcriptional activation domain of OCL1 was localized to 85 amino acids in the N-terminal part of the START domain. Full-length OCL1 devoid of this activation domain is unable to trans-activate a reporter gene under the control of a minimal promoter fused to six repeats of the L1 box, a cis-element present in target genes of HD-ZIP IV TFs in Arabidopsis. In addition, ectopic expression of OCL1 leads to pleiotropic phenotypic aberrations in transgenic maize plants, the most conspicuous one being a strong delay in flowering time which is correlated with the misexpression of molecular markers for floral transition such as ZMM4 (Zea Mays MADS-box4) or DLF1 (DELAYED FLOWERING1). As suggested by the interaction in planta between OCL1 and SWI3C1, a bona fide subunit of the SWI/SNF complex, OCL1 may modulate transcriptional activity of its target genes by interaction with a chromatin remodelling complex. © 2010 The Author.

Item Type: Paper
Uncontrolled Keywords: Activation domain chromatin remodelling flowering time maize START transcription factor
Subjects: organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic plants
CSHL Authors:
Communities: CSHL Post Doctoral Fellows
CSHL labs > Timmermans lab
Depositing User: CSHL Librarian
Date: 2011
Date Deposited: 09 Nov 2011 15:43
Last Modified: 02 May 2013 18:55
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15626

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