A Splicing-Independent Function of SF2/ASF in MicroRNA Processing

Wu, H., Sun, S., Tu, K., Gao, Y., Xie, B., Krainer, A. R., Zhu, J. (April 2010) A Splicing-Independent Function of SF2/ASF in MicroRNA Processing. Molecular Cell, 38 (1). pp. 67-77.

URL: http://www.ncbi.nlm.nih.gov/pubmed/20385090
DOI: 10.1016/j.molcel.2010.02.021

Abstract

Both splicing factors and microRNAs are important regulatory molecules that play key roles in posttranscriptional gene regulation. By miRNA deep sequencing, we identified 40 miRNAs that are differentially expressed upon ectopic overexpression of the splicing factor SF2/ASF. Here we show that SF2/ASF and one of its upregulated microRNAs (miR-7) can form a negative feedback loop: SF2/ASF promotes miR-7 maturation, and mature miR-7 in turn targets the 3′UTR of SF2/ASF to repress its translation. Enhanced microRNA expression is mediated by direct interaction between SF2/ASF and the primary miR-7 transcript to facilitate Drosha cleavage and is independent of SF2/ASF's function in splicing. Other miRNAs, including miR-221 and miR-222, may also be regulated by SF2/ASF through a similar mechanism. These results underscore a function of SF2/ASF in pri-miRNA processing and highlight the potential coordination between splicing control and miRNA-mediated gene repression in gene regulatory networks. © 2010 Elsevier Inc. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: PROTEINS RNA
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
CSHL Authors:
Communities: CSHL labs > Krainer lab
CSHL Cancer Center Shared Resources > Antibody and Phage Display Service
Depositing User: CSHL Librarian
Date: 9 April 2010
Date Deposited: 19 Oct 2011 19:56
Last Modified: 29 Dec 2014 20:55
PMCID: PMC3395997
Related URLs:
URI: http://repository.cshl.edu/id/eprint/15589

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving